Electrochemical magnetic beads-based immunosensing platform for the determination of α-lactalbumin in milk
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2016
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Elsevier BV
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Ruiz-Valdepeñas Montiel V, Campuzano S, Torrente-Rodríguez RM, Reviejo AJ, Pingarrón JM. Electrochemical magnetic beads-based immunosensing platform for the determination of α-lactalbumin in milk. Food Chem. 2016 Dec 15;213:595-601. doi: 10.1016/j.foodchem.2016.07.004. Epub 2016 Jul 5. PMID: 27451223.
Abstract
Alpha-lactalbumin (α-LA) is one of the whey proteins in cows’ milk that has been identified as allergenic. In this work, we present, for the first time, a very sensitive magnetic beads (MBs)-based immunosensor for the determination of α-LA. A sandwich configuration involving selective capture and horseradish peroxidase-labeled detector antibodies was implemented on carboxylic acid-modified magnetic beads, captured magnetically under the surface of a disposable screen-printed carbon electrode for amperometric detection using the hydroquinone (HQ)/hydrogen peroxide system. The α-LA immunosensor exhibited a wide linear range (37.0–5000 pg/ml), a low limit of detection (LOD, 11.0 pg/ml) and noteworthy selectivity against other non-target proteins. The MBs-based immunosensing platform was applied successfully for the determination of α-LA in several varieties of milk (raw and UHT cows’ milk as well as human milk) and infant formulations. The results were corroborated with those obtained using a commercial ELISA method, thereby substantiating the analytical merits of this unique method.