Uptake of nanoparticles by alveolar macrophages is triggered by surfactant protein A

Ruge, Christian Arnold; Kirch, Julian; Cañadas Benito, Olga; Schneider, Marc; Pérez Gil, Jesús; Schaefer, Ulrich Friedrich; Casals Carro, María Cristina; Lehr, Claus Michael

Uptake of nanoparticles by alveolar macrophages is triggered by surfactant protein A

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Official URL

https://www.sciencedirect.com/science/article/pii/S1549963411002863?via%3Dihub

Publication date

2011

Authors

Ruge, Christian Arnold

Kirch, Julian

Cañadas Benito, Olga

Schneider, Marc

Pérez Gil, Jesús

Schaefer, Ulrich Friedrich

Casals Carro, María Cristina

Lehr, Claus Michael

Publisher

Elsevier

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URI

https://hdl.handle.net/20.500.14352/94434

Citation

Ruge CA, Kirch K, Cañadas O, Schneider M, Pérez-Gil J, Schaefer UF, Casals C, Lehr CM. Uptake of nanoparticles by alveolar macrophages is triggered by surfactant protein A. Nanomedicine: Nanotechnology, Biology and Medicine 2011 Dec;7:690-693

Abstract

Understanding the bio-nano interactions in the lungs upon the inhalation of nanoparticles is a major challenge in both pulmonary nanomedicine and nanotoxicology. To investigate the effect of pulmonary surfactant protein A (SP-A) on the interaction between nanoparticles and alveolar macrophages, we used magnetite nanoparticles (110-180 nm in diameter) coated with different polymers (starch, carboxymethyldextran, chitosan, poly-maleic-oleic acid, phosphatidylcholine). Cellular binding and uptake of nanoparticles by alveolar macrophages was increased for nanoparticles treated with SP-A, whereas albumin, the prevailing protein in plasma, led to a significant decrease. A significantly different adsorption pattern of SP-A, compared to albumin was found for these five different nanomaterials. This study provides evidence that after inhalation of nanoparticles, a different protein coating and thus different biological behavior may result compared to direct administration to the bloodstream

Description

From the clinical editor: In this nano-toxicology study of inhaled nanoparticles, the authors investigated the effect of pulmonary surfactant protein A on the interaction between nanoparticles and alveolar macrophages utilizing magnetite nanoparticles coated with different polymers (starch, carboxymethyldextran, chitosan, poly-maleic-oleic acid, phosphatidylcholine). Cellular binding and uptake of nanoparticles increased for nanoparticles treated with SP-A, whereas albumin, the prevailing protein in plasma, led to a significant decrease.